Prepare all ingredients. Finely chop the truffle, break the chocolate into smaller pieces, separate the eggs, placing the egg whites in a clean bowl.
Mix the egg yolks with the sugar, vanilla sugar, and chopped truffle.
Pour the milk into a saucepan and heat over medium heat until lukewarm. Then, pour the warm milk into the egg yolk mixture.
Place the chocolate and butter in a small bowl and melt in a double boiler. Once the chocolate is melted, pour it into the egg yolk mixture and mix well.
Whisk the egg whites until stiff peaks form, and gently fold them into the chocolate mixture.
Sift the flour and gradually add it to the chocolate mixture. Mix well to combine.
Grease a soufflé dish with butter, then pour the batter into the prepared dish.
Bake in a preheated oven at 350°F (180°C) for 20-25 minutes, or until the soufflé has risen nicely and is golden brown.
Once baked, let it cool slightly before serving warm.
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